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Proteins’ Fluorescence A Little Less Mysterious（图）
Proteins Fluorescence Little Less Mysterious
2018/2/1
Rice University scientists have effectively quenched a debate over the mechanism behind a fluorescent biosensor that monitors neurons by sensing changes in voltage.The work led by Rice theoretical che...
Anomalous Negative Fluorescence Anisotropy in Yellow Fluorescent Protein (YFP 10C):Quantitative Analysis of FRET in YFP Dimers
Alzheimer's disease Amyloid beta 40 peptide Human serum albumin Circular dichroism Dissociation constant alpha-Helix
2016/5/25
Yellow fluorescent protein (YFP) is widely used as a genetically encoded fluorescent marker in biology. In the course of a comprehensive study of this protein, we observed an unusual, negative fluores...
Enhancement of the Fluorescence of the Blue Fluorescent Proteins by High Pressure or Low Temperature
Enhancement Fluorescence Blue Fluorescent Proteins High Pressure Low Temperature
2016/5/24
Green fluorescent proteins bearing the Y66H mutation exhibit strongly blue-shifted fluorescence excitation and emission spectra. However, these blue fluorescent proteins (BFPs) have lower quantum yiel...
Probing the Structure of Supported Membranes and Tethered Oligonucleotides by Fluorescence Interference Contrast Microscopy
Water Oligonucleotides Microscopy, Fluorescence Microscopy, Interference Membranes, Artificial Sensitivity and Specificity Surface Properties Models, Chemical Silicon Dioxide
2016/5/24
Fluorescence interference contrast microscopy (FLIC) is a powerful method to structurally characterize fluorescent objects with nanometer-scale resolution in the z direction. Here we use FLIC to chara...
Early Steps of Supported Bilayer Formation Probed by Single Vesicle Fluorescence Assays
Adsorption Diffusion Lipid Bilayers Lipid Bilayers Liposomes Membrane Fluidity Membrane Fusion Membranes,Artificial Microscopy,Fluorescence,Multiphoton Motion Phosphatidylcholines Phosphatidylserines Xanthenes
2016/5/24
We have developed a single vesicle assay to study the mechanisms of supported bilayer formation. Fluorescently labeled, unilamellar vesicles (30-100 nm diameter) were first adsorbed to a quartz surfac...
The Effect of Very High Magnetic Fields on the Delayed Fluorescence from Photosynthetic Reaction Centers
Reactioncenter Photosynthesis Bacterialphotosynthesis Delayedfluorescence Magneticfieldeffect (Rb. sphaeroides)
2016/5/23
Delayed fluorescence is observed from quinone-depleted Rhodobacter sphaeroides reaction centers due to re-formation of the excited state of the special pair electron donor from the initially formed ra...
Electric Field Modulation of the Fluorescence Spectrum from Rhodobacter Sphaeroides Reaction Centers
fluorescence counterstain nuclear counterstain immunocytochemistry propidium iodide cell culture
2016/5/20
The fluorescence intensity from randomly oriented Rhodobacter ( Rb.) sphaeroides photosynthetic reaction centers at 77 K increases in an applied electric field. This is ascribed to a net reduction in ...
Fluorescence Lifetimes Measurements Using a Synchrotron Source
Fluorescence Lifetimes Measurements Synchrotron Source
2016/5/20
Fluorescence Lifetimes Measurements Using a Synchrotron Source.
The SOS response in Escherichia coli: Single cell analysis using fluorescence microscopy
Biological sciences DNA repair RecA SOS response
2015/1/28
During the course of DNA replication, replication forks often stall or collapse as they proceed from oriC to the terminus due to housekeeping types of DNA damage or proteins bound to DNA. If the DNA i...
Characteristic spectral features of the polarized fluorescence of human breast cancer in the wavelet domain
Florescence Spectroscopy Wavelet Transform Biomedical Optics Breast Cancer
2012/5/17
Wavelet transform of polarized fluorescence spectra of human breast tissues is found to localize spectral features that can reliably differentiate normal and malignant tissue types. The intensity diff...
应用双色荧光原位杂交技术检测克氏综合征The Application of Dual-color Fluorescence in situ Hybridization to the Diagnosis of Klinefelter Syndrome
荧光原位杂交 克氏综合征 性染色体异常 DNA特异性探针
2007/12/30
摘要正常对照标准值4.17%）。用FISH 技术检测性染色体数目异常克氏综合征具有快速、敏感度高、信号强、背景低、多色等优点，故FISH 技术在产前诊断检测领域中显示其重要的应用价值和发展前景。
Abstract:The objective of the work is to study the technique of dual-color fluorescence in situ hybri...
Rapid Sex Identification of Chicken by Fluorescence In Situ Hybridization Using a W Chromosome-specific DNA Probe
Chicken W Chromosome XhoI family PCR FISH Sexing
2016/4/12
It has been known that the sex of chicken cells can be most accurately identified by fluorescence in situ hybridization (FISH). However, the presently available FISH has not been widely used for sex i...