搜索结果: 1-15 共查到“农学 real time-PCR”相关记录17条 . 查询时间(0.14 秒)
Real-Time PCR Applied to Study on Plant Pathogens: Potential Applications in Diagnosis – a Review
bacteria fungi oomycetes phytoplasmas viroids viruses plants
2015/10/9
Quantitative real-time PCR (qPCR) technique incorporates traditional polymerase chain reaction (PCR) efficiency
with the production of a specific fluorescent signal, measuring the kinetics of the re...
Real time PCR quantification of Sclerotium rolfsii in chilli tissue and soil
chilli root rot specific primer pair pathogen identification population detection population determination
2015/7/28
Root rot disease caused by Sclerotium rolfsii is one of the important factors limiting chilli production in southern China. Rapid methods of detection and identification of S. rolfsii are required for...
Evaluation of reference genes for gene expression studies in pig muscle tissue by real-time PCR
gene stability reference genes quantitative PCR Sus scrofa age categories of pigs muscle tissue
2015/6/9
The selection of reference genes is essential for gene expression studies when using a real-time quantitative polymerase chain reaction (PCR). Reference gene selection should be performed for each exp...
Evaluation of the microbial population in ruminal fluid using real time PCR in steers treated with virginiamycin
virginiamycin roll-tube technique ruminal fermentation parameters real-time PCR
2015/6/8
The objective of the present study was to investigate the effects of virginiamycin (VM ) supplementation on ruminal fermentation and microbial populations in steers. Four ruminally cannulated Chinese ...
A simplified duplex real-time PCR incorporating TaqMan minor groove binder (MGB) probes and an exogenous internal positive control for the simultaneous detection of Mycoplasma gallisepticum and Mycoplasma synoviae cultures
avian Mycoplasma spp duplex real-time PCR
2015/5/25
Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) are the most pathogenic and
economically important mycoplasma pathogens that infect chickens. The development of rapid and innovative
mol...
Recovery of Cryptosporidium from spiked water and stool samples measured by PCR and real time PCR
Cryptosporidium parvum oocysts spiked water and stool water filtration
2015/4/16
Cryptosporidium parvum is a common intestinal protozoan parasite infecting humans and a wide range of animals, whose diagnostics present considerable difficulties. These arise from the exceptionally r...
Mycobacterium avium subsp. paratuberculosis in powdered infant milk: F57 competitive real time PCR
Crohn's disease public health food safety environmental risk
2015/4/15
Mycobacterium avium subsp. paratuberculosis (MAP) in concentrations from 48 to 32 500 cells per gram of powdered infant milk were found in 18 out of 51 investigated samples (35%) in this study. More t...
Mycobacterium avium subsp. hominissuis infection in two sibling Fjord horses diagnosed using quantitative real time PCR: a case report
Mycobacterium avium complex mycobacteriosis qPCR IS1245 zoonosis
2015/4/15
This report describes new possibilities for intravital and post mortem diagnosis of avian mycobacteriosis in horses using the quantitative real time PCR (qPCR) method. Using this method, Mycobacterium...
Examination of Mycobacterium avium subsp. avium distribution in naturally infected hens by culture and triplex quantitative real time PCR
chicken mycobacteriosis non-tuberculous mycobacteria
2015/4/7
Mycobacterium avium subsp. avium (MAA) is the etiologic agent of avian tuberculosis, a chronic contagious disease described in a wide variety of domestic and wild bird species. The aims of this study ...
Mycobacterium avium subsp. paratuberculosis in a mouflon herd without clinical symptoms monitored using IS900 real-time PCR: a case report
MAP Johne's disease faeces
2015/4/7
The aim of this study was to monitor over two years a farmed mouflon herd for the presence and persistence of Mycobacterium avium subsp. paratuberculosis (MAP) using an IS900 real-time PCR method. Thi...
Real-time PCR for quantitation of bovine viral diarrhea virus RNA using SYBR Green I fluorimetry
bovine viral diarrhea virus RNA real-time RT-PCR SYBR Green I
2015/3/13
Quantitative real-time RT-PCR (qRT-PCR) assay was developed for the detection and quantification of bovine viral diarrhea virus (BVDV) in clinical samples from persistently infected cattle. qRT-PCR wa...
Quantification of Tilletia caries and Tilletia controversa Mycelium in Wheat Apical Meristem by Real-time PCR
Tilletia spp real-time PCR apical meristem absolute quantification
2015/3/5
In the Czech Republic, three closely related species of the genus Tilletia belong to pathogens that cause significant losses of wheat crops by replacing grains with a mass of teliospores. A quantitati...
本研究旨在揭示感染JSRV病羊体内检测不到循环抗体的免疫学机理。用地高辛（DIG）标记制备enJSRV-env探针，原位杂交法检测妊娠70 d的绵羊胎儿（免疫器官初步形成期）、妊娠130 d胎儿（即将出生的）和出生7日龄羔羊的胸腺、脾脏、肠淋巴结和肺脏内enJSRV mRNA表达情况。并利用Real-Time PCR法，对enJSRV mRNA在以上组织中的表达进行定量分析。结果表明，在所检测组织...
采用实时荧光定量PCR方法检测了不同感光大豆品种的CO(CONSTANS) 基因在不同光照条件下的mRNA水平变化。结果表明：早熟品种东农49和晚熟品种东农42各自在短日照(8h/16h 光/暗)下会比长日照(16 h/8 h 光/暗)下表达有所增强，同时东农49中CO基因的表达要高于东农42，它们的表达峰值均出现在光暗交界处。东农42由长日照或短日照移入完全光或完全暗条件时，暗下CO基因的表达量...
[ 目的] 为转基因番茄植株的高通量筛选奠定基础。[ 方法] 利用CTAB 法提取番茄叶片总RNA 进行Real Time PCR 扩增, 分析转Mi
1 .2 基因的番茄植株表达水平的检测体系。[ 结果] 提取RNA 的A260/ A280 为1 .78 ～1 .88 ,RNA 无明显降解。在严谨扩增条件下, 引物SYBR2 的扩增效率高于SYBR1 。Mg2 + 的适宜浓度为2 .0 mg/...